Sorawuth Bunthawin, Puwadon Butrat and Maneerat Boonlamp

pp. 227 - 237

Abstract

This research developed a computer program to calculate electrical properties of a cell from a spherical single shell model. The program compared the results between the theoretical values (Re[f(ω)]_TDS) and
the experimental values (Re[f(ω)]_EDS). The latter was computed from a cell velocity obtained from dielectrophoresis. The calculation was repeated until the error percentile was in a proper range. The program was
applied to process the electrical properties of Phuket pineapple protoplasts [Ananas comosus (L.) Merr.C.V.
Phuket]. It was found that the thickness of the cell membrane was 10 nm, the dielectric constants of suspending solution, cytoplasm and cell membrane were 80ε₀, 58-60ε₀ and 10-14ε₀ respectively (ε₀ is the dielectric
constant of the vacuum = 8.85×10^-12 F m^-1). The conductivity of cytoplasm and cell membrane were 0.09 S
m^-1 and 10^-5 - 10^-4 S m^-1 respectively

Keywords

• computer program
• conductivity
• dielectrophoresis
• dielectric constant
• Phuket pineapple
• single-shell model