Kanyarat Srihaphon, Supaporn Lamlertthon and Tasana Pitaksuteepong

DOI: 10.14456/sjst-psu.2021.10

pp. 72 - 79

Abstract

This study aimed to investigate the effects of stabilizers and cryoprotectants on the characteristics of poly (lactic-coglycolic) acid (PLGA) nanoparticles containing Morus alba stem extract (MSE). The stabilizers used in the preparation of the
nanoparticles were polyvinyl alcohol (PVA) and poloxamer 188 at a concentration of 2% w/v. The effects on freeze-drying of
cryoprotectants, including glucose, sucrose, maltose and mannitol at concentrations of 5% and 10% w/v, were investigated. The
results showed that the presence or the absence as well as the type of stabilizer affected size and charge of the nanoparticles.
They also affected the loading capacity and the release of MSE. In freeze-drying, the type of cryoprotectant greatly influenced
the physical stability of the nanoparticles and the chemical stability of encapsulated MSE. The nanoparticles prepared using 2%
w/v PVA as the stabilizer and freeze-dried in the presence of 5% sucrose were the optimal carriers of MSE. 

Keywords

• Morus alba stem extract
• PLGA
• nanoparticles
• stabilizer
• cryoprotectant