A metabolomics workflow is used to trace the connection between the chemical allocation and the bud differentiation in the sponge Penares cf. nux. The sponge specimens collected from two locations; Koh-Tao, Surat-Thani province, and Saiburi, Pattani province, were separated into two parts, the budding capitums and the bases. The geographic locations had no apparent effects on the sponge’s chemical profiles. PCA of the CHCl3-extracts, however, discriminated the capitums from the bases, and emphasized the impacts from the trisoxazole macrolides and the sterols. The results indicate that the sponge was able to allocate the secondary metabolites towards the capitums. On the other hand, the dataset of the aq MeOH-extracts, representing the watersoluble primary metabolites, was scattered throughout the score plot and suggests that there were no differences between the primary metabolisms in the two parts.
