Somchai Chuan-udom, Mongkol Sae-Lim and Mii Masahiro

pp. 465 - 472

Abstract

DNA amplification (RAPD), sequencing (RAPD-based PCR) and quantification (flow cytometry)
were tested for their ability to identify Lansium spp.; duku, langsat and longkong. The results revealed that
all the techniques tested can be used successfully. It was clearly seen that DNA amplification and sequencing
are cumbersome, expensive and time consuming. Flow cytometry is simple and quick to use, less labour
intensive and accurate/repeatable and hence can be considered a practical alternative to the others. The data
for measurement of DNA content showed that duku has the lowest DNA content while longkong has the
highest DNA content and langsat was intermediate.

Keywords

• cultivar identification
• Lansium spp.
• PCR
• RAPD
• DNA sequencing
• flow cytometry
• phylogenetic relationship