Original Article | 2006, Vol.28, No.4, pp. 717-722
Cultivar, explant type and culture medium influencing embryogenesis and organogenegenesis in Anthurium spp.
Somchai Chuan-udom, Thanyaporn Susanon and Yaowaphan Sontikun
pp. 717 - 722
Abstract
The effect of cultivars, culture media, explant type and wounding on culture response of anthurium were studied. Valantino gave the highest callus formation (83.73%) significantly different from Sonat (78.73%) and Plew Thien Phuket (45.66%). Valantino and Plew Thien Phuket gave meristematic nodular callus (MNC) whereas Sonat produced embryogenic-like callus (ELC). Modified Murashige and Skoog (MMS) medium gave the highest callus formation from both leaf (86.6%) and node (100%). Callus obtained in Nitsch and Nitsch (NN) and MMS was MNC while woody plant (WPM) medium provided ELC. For explant types, internode gave the highest callus formation (72.63%). Nodal and internodal explant gave ELC while the leaf explant yielded MNC. Wounding leaf blades tended to promote more MNC.
