Original Article | 2007, Vol.29 Supplementary II : Grad. Res., pp.237-246
Plantlet regeneration and somaclonal variation in Anthurium cv. Sultan through embryogenesis
Yaowaphan Sontikul and Somchai Chuan-udom
pp. 237 - 246
Abstract
Calli of anthurium cv. Sultan were cultured on liquid or solid MMS (modified Murashige and Skoog) medium. The solid medium was supplemented with 1.0-4.0 mg/l 2,4-D (2,4-dichlorophenoxyacetic acid) in combination with 0.5 and 1.0 mg/l kinetin or 0.5, 0.75 and 1.0 mg/l TDZ (thidiazuron) with 0.5 and 1.0 mg/l BA (N6-benzyladenine) for 16 weeks. The result showed that a cluster of embryogenic callus (1.3-1.5 cm) was formed on medium with 2.0 mg/l 2,4-D and 0.5 mg/l kinetin (90%) and developed into shoot and shoot with root (83.3% and 100%, respectively). The solid medium with 1.0 mg/l TDZ and 1.0 mg/l BA gave 66.67% embryogenic callus formation after culturing for 12 months. Liquid medium supplemented with 3.0 mg/l 2,4-D and 0.5 mg/l BA gave the best fresh weight of embryogenic callus (3.33 g). Regenerants obtained from BA and TDZ containing solid medium produced abnormal lanceolate leaves. Isozyme markers revealed different pattern of esterase activity in normal and abnormal leaves.
