Ornubol Chomdej, Orawan Chatchawankanpanich, Wichai Kositratana and Julapark Chunwongse

pp. 1469 - 1477

Abstract

Tomato germplasm accessions; FLA456-4, FLA591-15, H24, CLN2443A, CLN2443B, CLN2443C, TLB111, TLB182-1, TLB111-F6-4-1, TLB130-F6-3-1 and TLB134-F6-8-1 from the Asian Vegetable Research Development Center (AVRDC), Taiwan, were screened for resistance to the Tomato Yellow Leaf Curl Virus, Thailand isolate (TYLCTHV-[2]). The accessions expressing the resistant genotype were then crossed to the TYLCV-susceptible female parent, Seedathip3 (SD3), to produce F1 hybrids. Tomato parents and their F₁ progenies were inoculated with TYLCTHV-[2] at 3 weeks of seedling age using viruliferous whitefly (Bemisia tabaci) as the inoculation vector. Disease response of the seedling was rated according to the incidence and severity of the development of yellowing and curling symptoms. The presence of TYLCTHV-[2] in the inoculated plants was confirmed by Enzyme-Linked Immunosorbent Assay (ELISA). AVRDC tomato parental lines: H24, FLA591-15 and FLA456-4 expressed mild or no symptoms after one month inoculation. Progeny of crosses between the AVRDC donor parental lines and susceptible Thai cultivars showed intermediate tolerance to TYLCTHV-[2] infection. This indicated that resistance was incompletely dominant.

Keywords

• geminivirus
• Lycopersicon esculentum
• tomato yellow leaf curl disease
• TYLCTHV-[2]
• breeding for resistance