Original Article | 2010, Vol.32, No.6, pp. 555-559
CDNA library from the Latex of Hevea brasiliensis
Wilaiwan Chotigeat, Sarapee Duangchu and Amornrat Phongdara
pp. 555 - 559
Abstract
Latex from Hevea brasiliensis contains 30-50% (w/w) of natural rubber (cis-1,4-polyisoprene), the important raw material for many rubber industries. We have constructed a cDNA library from the latex of H. brasiliensis to investigate the expressed genes and molecular events in the latex. We analyzed 412 expressed sequence tags (ESTs). More than 90% of the EST clones showed homology to previously described sequences in public databases. Functional classification of the ESTs showed that the largest category were proteins of unknown function (30.1%), 11.4% of ESTs encoded for rubber synthesisrelated proteins (RS) and 8.5% for defense or stress related proteins (DS). Those with no significant homology to known sequences (NSH) accounted for 8.7%, primary metabolism (PM) and gene expression and RNA metabolism were 7.8% and 6.6%, respectively. Other categories included, protein synthesis-related proteins (6.6%), chromatin and DNA metabolism (CDM 3.9%), energy metabolism (EM 3.4%), cellular transport (CT 3.2%), cell structure (CS 3.2%), signal transduction (ST 2.2%), secondary metabolism (SM 1.7%), protein fate (PF 2.2%), and reproductive proteins (RP 0.7%).
