Suphat Rittirat, Somchai Chuan-udom, Nattaporn Kerdsuwan and Soisiri Kongruk

pp. 659 - 663

Abstract

Efficient propagation of Chang Daeng through embryogenic callus was investigated. Young shoot (approx. 10 mm)
gave the highest percentage of multiple shoot formation (100), number of shoots (10 shoots/explant) and embryogenic callus
(EC) formation (56%) Proliferation of the calli in both Vacin and Went (VW) and New Dogashima medium (NDM) supplemented with 2% sucrose and 15% coconut water (CW) under light or dark condition was nearly the same. The color of calli
in VW in both conditions was light yellow or cream while NDM provided bright yellow or yellowish green. Calli subcultured
on NDM medium under light condition turned to green and then produced the highest percentage formation (79.8) and
number of mature somatic embryos (9 embryos/callus). Within one month, those embryos germinated in the presence of AC
in the culture medium. The shoots at length more than 3 cm with an average number of 2-3 roots were successfully transferred
to soil. This empirical technique could be useful for micropropagation as well as genetic transformation in this plant. 

Keywords

• Chang Daeng
• Rhynchostylis rubrum
• micropropagation
• embryogenic callus