Original Article | 2012, Vol.34, No.6, pp. 623-628
Established GC-FID for simultaneous determination of diterpenes and phytosterols in Plaunoi(Croton stellatopilosus Ohba)
Damrong Kongduang, Juraithip Wungsintaweekul and Wanchai De-Eknamkul
pp. 623 - 628
Abstract
A gas chromatography-flame ionization detector (GC-FID) was established and validated for simultaneous determination of the diterpenes (phytol, GGOH, plaunotol) and phytosterols (campesterol, stigmasterol and β-sitosterol) in plant leaves, callus and suspension cultures of Plaunoi (Croton stellatopilosus). Under the developed GC-FID conditions, the diterpenes and phytosterols were separated in a single run. The limit of quantification (LOQ) and the limit of detection (LOD) of each standard sample were determined. Interday and intraday precisions were less than 5%. The average recoveries of the method were 102.4-109.9% and all compounds showed linearity with r2 values all ≥ 0.9994. According to this newly established GC-FID method, the metabolites that accumulated in the leaves, green callus and suspension cultures of Plaunoi were determined. Phytol, GGOH and plaunotol were present at relatively high concentrations in plant leaves, and in considerably reduced amounts in green callus cells. Phytol and plaunotol were not detected in suspension cell cultures.
