Total phenolic content, antioxidant and antimicrobial activities of Blepharis edulis extracts
Mohaddese Mahboubi, Ghasem Haghi, Nastaran Kazempour and Ali Reza Hatemi
pp. 11 - 16
Abstract
Blepharis edulis is traditionally used as an antiseptic, purgative, aphrodisiac and anti-inflammatory agent. The extracts of plant aerial parts were screened for total phenolic content (TPC) gallic acid equivalents (GAE), total flavonoid compound (TFC) quercetin equivalents (QE), antioxidant capacity and its antimicrobial activity by micro broth dilution assay. The 50%- inhibition values of BHT and 70% (v/v) aqueous ethanol, 70% (v/v) aqueous methanol, methanol, and water extracts of B. edulis according to the DPPH method were found to be 19.6, 71.2, 73.7, 81.4, and 218.4 μg/ml, respectively. TPC ranged from 38.9 to 102.7 mg GAE/g dry extracts. The antimicrobial activity showed that yeast and fungi were sensitive and resistant microorganisms to the extracts. The 70%-methanol extract showed more drastic antimicrobial activity than the others. The antimicrobial activity of ethanolic extract is the same as of the methanolic extract; water extract had the weakest antimicrobial activity.
