Original Article | 2013, Vol.35, No.2, pp. 149-158
Development of EST-derived markers in Dendrobium from EST of related taxa
Narisa Juejun, Chataporn Chunwongse and Julapark Chunwongse
pp. 149 - 158
Abstract
Public databases are useful for molecular marker development. The major aim of this study was to develop expressed sequence tag (EST)-derived markers in Dendrobium from available ESTs of Phalaenopsis and Dendrobium. A total of 6063 sequences were screened for simple sequence repeats (SSRs) and introns. Primers flanking these regions were generated and tested on genomic DNAs of Phalaenopsis and Dendrobium. Twenty-three percent of amplifiable Phalaenopsis EST-derived markers were cross-genera transferable to Dendrobium. Forty-one markers from both Phalaenopsis and Dendrobium that amplified in Dendrobium were assessed on six commercial cultivars and six wild accessions. All of them were transferable among Dendrobium species. High polymorphism and heterozygosity were observed within wild accessions. Sixteen polymorphic markers were evaluated for linkage analysis on an F1 segregating population. Seven markers were mapped into three linkage groups, two of which showed syntenic relationship between dendrobium and rice. This relationship will facilitate further quantitative trait loci (QTL) mapping and comparative genomic studies of Dendrobium. Our results indicate that Phalaenopsis EST-derived markers are valuable tools for genetic research and breeding applications in Dendrobium.
