Md. Fakruddin, Khanjada Shahnewaj Bin Mannan, Reaz Mohammad Mazumdar, Abhijit Chowdhury and Md Nur Hossain

pp. 397 - 404

Abstract

Identification and classification of the microorganisms are of utmost importance in the field of environmental, industrial,
medical and agricultural microbiology, and microbial ecology. Traditional phenotype-based methods encounter many
challenges and shortcomings which limit their usability. Molecular methods offer better solutions in identifying and characterizing microorganisms. Several DNA fingerprinting methods have been developed and are in use already. In principle, most
of these methods are based on PCR and restriction site analysis. Some of these methods are still not economic in use and
require huge set-up cost. Continuous research is going on around the world to improve the methodology and applicability of
these methods as well as to make them economic in use.

Keywords

• fingerprinting
• RFLP
• SSCP
• RAPD
• MLST
• PFGE