Original Article | 2013, Vol.35, No.5, pp. 537-546
Wound-healing potential of grandiflorenic acid isolated from Wedelia trilobata (L.) leaves
Neelam Balekar, Titpawan Nakpheng and Teerapol Srichana
pp. 537 - 546
Abstract
The ethyl acetate fraction from ethanolic extract of Wedelia trilobata (L.) leaves displayed wound healing properties. The ethyl acetate fraction was further subjected to bioassay-guided fractionation which afforded isolation of grandiflorenic acid which requires further investigation to prove its wound healing potential. The grandiflorenic acid from leaves of Wedelia trilobata was assessed for its possible activity on BJ human fibroblast and HaCaT keratinocytes proliferation, and effect on in vitro scratch assay, collagen content, TGF-β2 levels, and nitric oxide, TNF-α and IL-1β determination using Raw 264.7 cells. Grandiflorenic acid (2.5µg/mL) produced percentage viability of BJ human fibroblast, and HaCaT keratinocytes 116, and 106% respectively. Grandiflorenic acid (2.5 µg/mL) induced a 100% migration rate in the in vitro scratch assay and the collagen content was increased to 171.2 µg/mL compared to the control (61.1µg/mL) with BJ human fibroblast. Grandiflorenic acid (2.5µg/mL) neither produced any significant increase in TGF-β2 levels of HaCaT keratinocytes cells nor induced migration of HaCaT cells in the in vitro scratch assay. The present study provides scientific evidence that grandiflorenic acid has potential wound healing activity due to combination of fibroblast stimulation and inhibiting prolonging inflammatory phase of wound healing evident by reduced levels of inflammatory cytokines from macrophage Raw 264.7 cells.
