Original Article | 2013, Vol.35, No.6, pp. 659-664
Anti-HIV-1 integrase and anti-allergic activities of Bauhinia strychnifolia
Kingkan Bunluepuech, Chatchai Wattanapiromsakul, Fameera Madaka and Supinya Tewtrakul
pp. 659 - 664
Abstract
A stem ethanol extract of Bauhinia strychnifolia and its compounds were investigated for their anti-HIV-1 integrase (IN) and anti-allergic activities. From bioassay-guided isolation, five compounds including quercetin (1), 3,5,7,3',5'- pentahydroxyflavanonol-3-O-α-L-rhamnopyranoside (2), 3,5,7-trihydroxychromone-3-α-L-rhamnopyranoside (3) and a mixture of β-sitosterol (4) and stigmasterol (5) were isolated. Of the tested samples, compound 1 (quercetin) showed the highest activity against HIV-1 IN with an IC50 value of 15.2 µM, followed by 3 (3,5,7-trihydroxychromone-3-α-Lrhamnopyranoside), 4+5 (mixture of β-sitosterol and stigmasterol) and 2 (3,5,7,3',5'-pentahydroxyflavanonol-3-O-α-Lrhamnopyranoside) with % inhibition of 28.2, 26.2 and 6.7 at 100 µM, respectively. With regard to anti-allergic activity, quercetin (1) possessed the highest anti-allergic activity with an IC50 of 8.1 µM, followed by 3 (3,5,7-trihydroxychromone-3- α-L-rhamnopyranoside) and 4+5 (mixture of β-sitosterol and stigmasterol) with IC50 values of 52.1 and 77.5 µM, respectively. Compound 2 (3,5,7,3',5'-pentahydroxyflavanonol-3-O-α-L-rhamnopyranoside) was inactive. The present study is the first report of chemical constituents and biological activities of Bauhinia strychnifolia.
