Kulchanat Prasertsit, Thitipong Rugwong and Pakamas Chetpattananondh

pp. 353 - 359

Abstract

A large amount of jackfruit seeds can be extracted for possible prebiotics and phenolics (gallic acid) and purified by
crystallization and solid phase extraction (SPE), respectively. The studied parameters for crystallization were mixing speed,
crystallizing temperature and cooling rate while the studied parameters for SPE were feed concentration, feed flow rate and
length to diameter ratio (L/D) of the extraction column. The optimal condition for the crystallization provided the crystal yield
of 0.028 g crystal /g dry extract at 100 rpm mixing speed, 58°C crystallizing temperature and 1°C /min cooling rate. Molecular
weight analysis of the crystals indicated that the crystals can be fructo-oligosaccharide or raffinose. The optimal condition
for SPE obtaining gallic acid 92 percent yield was at 4% w/v feed concentration with 3 mL/min flow rate and L/D of 15.3.
Finally a high temperature can degrade both possible prebiotic and gallic acid.

Keywords

• prebiotics
• gallic acid
• jackfruit seeds
• crystallization
• solid phase extraction