Sunderamurthe Satisvar, Subash Chandra Bose Gopinath, Mohd Khairuddin Md Arshad, Dee Vie Ong, Thangavel Lakshmipriya, Chun Hong Voon, Uda Hashim, Abdul Rahim Ruslinda and Suresh Venkata Chinni

DOI: 10.14456/sjst-psu.2017.41

pp. 373 - 381

Abstract

In tandem to the Salmonellosis infection worldwide, a study was conducted to determine the conserved and nonconserved region in Salmonella enterica sub-species so that it can be used to design probes in biosensors for the detection
of Salmonella enterica as a species or sub-species distinctively. The region was selected for this study through fliC gene
that is present in all Salmonella sub-species, encodes the Salmonella flagella or flagellin determines the serotype due to
its H antigen and provides virulence to the bacteria. All sub-species were analyzed in a group of six and then analyzed
individually in groups of two with all possible combinations to determine the overlapping regions. Based on the study, the
predominant conserved region for S. enterica sub-species is between 103 and 158, whereas the non-conserved region is from
1245 to 1285. A phylogenetic tree was constructed for the S. enterica sub-species to determine the evolution of the
Salmonella sub-species. 

Keywords

• Salmonella enterica
• sub-species
• fliC gene
• flagellin
• H antigen
• biosensor