Somporn Prasertsongskun

pp. 637 - 642

Abstract

The present research aimed to establish cell suspension culture of vetiver (Vetiveria zizanioides Nash)
from Surat Thani germplasm source and efficient plant regeneration from callus derived from such cultures.
Cell suspension cultures were established from calli derived from inflorescence of vetiver. Optimum cell
proliferation occurred in liquid N₆ medium supplemented with 10 µM 2,4-dichlorophenoxyacetic acid (2,4-D)
and 10 mM proline. The cell suspension formed the highest small colonies when plated on solid MS medium
containing 0.45 µM 2,4-D. After subsequent transfer to regeneration medium (MS free medium) 65% of
plantlets were obtained.

Keywords

• regeneration
• callus
• vetiver
• Vetiveria zizanioides