Alagu Thirumurugan, Rajajurn Sheela and Amaresh Kumar Singh

DOI: 10.14456/sjst-psu.2019.5

pp. 37 - 44

Abstract

In this study, we report on the growth profile and partial characterization of a bacteriocin produced by Lactobacillus
plantarum ATM11 isolated from goat slaughterhouse soil. Among eight bacteriocin-producing bacteria from 25 Lactobacilli
isolates of goat slaughterhouse soil, the higher-yielding bacteria were selected and identified as Lactobacillus plantarum based on
the morphology, biochemical characteristics, and partial 16S rRNA gene sequence. The physico-chemical condition effects of
temperature, pH, enzymes, and detergents of bacteriocin activity were studied. The optimum temperature for bacteriocin activity
was found to be 60 C. The optimum pH for bacteriocin activity was found to be in the range of 5.0-6.0. Detergents such as
sodium dodecyl sulphate (SDS), Triton X-100, and ethylene diamine tetra-acetic acid (EDTA) were influenced the bacteriocin
activity, whereas urea inhibited the activity. Asparaginase and -amylase enzymes influenced the activity, whereas protease,
proteinase K, and lysozyme inhibited bacteriocin activity. The present study revealed that the action mode of the produced
bacteriocin has a bacteriostatic effect against Bacillus cereus MTCC 1272 and Micrococcus leuteus MTCC 2987. Thus, it can be
used as a bio-preservative agent for different foods in the future. 

Keywords

• bacteriocin
• biochemical tests
• 16S rRNA sequencing
• L. plantarum ATM11
• growth profile
• physicochemical characterization