This study aimed to investigate the effects of three different semen extenders (TEY, AND®, and TSY) and three equilibration periods (2, 4, and 6 h) on the storage of Bali bull spermatozoa. This research used a completely randomized block design with two factors (extenders and equilibration time) and 12 replications. The measured variables were fresh semen quality of Bali cattle, motility, viability, abnormality, plasma membrane integrity (PMI), recovery rate (RR), pregnancy rate (PR), and NRR. The results showed that the spermatozoa of the extended semen with TEY and AND® within 4 h equilibration period was able to show better motility, viability, PMI, and RR than the spermatozoa of the extended semen with TSY within 2-h and 6-h equilibration periods. The conclusion of this research is that the TEY, AND®, and TSY extenders on Bali bull semen with a 4-h equilibration time yielded 90% PR and 100% NRR.
