Genet Minale, Prapapan Temkitthawon, Neti Waranuch, Nitra Nuengchamnong, Ngamrayu Ngamdokmai, Supaporn Pitiporn, Pakakrong Kwankhao and Kornkanok Ingkaninan

DOI: 10.14456/sjst-psu.2021.95

pp. 719 - 728

Abstract

Centotheca lappacea (L.) Desv. or “hee yum” grass has been used in Thailand for postpartum care. This study focused
on analyses of elements, antioxidants and volatile compounds from C. lappacea. Wavelength dispersive X-ray fluorescence
showed the presence of silicon in this plant. Liquid chromatography–electrospray ionization-quadrupole-time of flight-mass
spectrometer coupled with DPPH assay was used for separation, detection and identification of antioxidants in C. lappacea in
one run. Twelve antioxidants including C-and O-glycosides of luteolin and apigenin were identified. Moreover, a headspace gas
chromatography-mass spectrometer was used for analyses of volatile compounds by direct burning of the plant material, which is
similar to how smoke is made during a traditional treatment. Nineteen and twenty-five compounds were found from the roots and
the aerial parts of C. lappacea, respectively. In conclusion, antioxidants and volatile constituents after C. lappacea has been burnt
are reported here for the first time.

Keywords

• Centotheca lappacea
• antioxidants
• LC-ESI-QTOF-MS
• DPPH
• headspace GC-MS