Azolla pinnata belongs to the family Salviniaceae. It has shown various beneficial effects in animals of lower phylogenetic order in curing hepatotoxicity. The main objective of the current study is, that one could add additional value to species holding great medicinal qualities. The different extractants tested were ethanol, methanol and water. The dilutions were varied among 1:32, 1:16, 1:8, 1:4, 1:2 and these were subjected to different pharmacological tests like Hot plate test, analgesic, and antipyretic tests. The different concentrations of Azolla pinnata were subjected to various assays like DPPH assay, peroxidase activity, and catalase activity. The surface morphology of Azolla pinnata was analyzed using Scanning Electron Microscope to potentially detect morphological features of its ultrastructure. Spectroscopy was performed to analyze the compositions of various components present in the plant. It was concluded that the ethanolic extract had very significant potential, higher than the rest of the extracts.
