Genetic variations among eight Pakistani tomato (Solanum lycopersicum L.) accessions were investigated using morphological traits and randomly amplified polymorphic DNA (RAPD) primers. Significant differences among accessions were observed for most of the quantitative and qualitative morphological traits. The RAPD-PCR assay amplified a total of 39 different alleles with 33 polymorphic alleles resulting in an 84.25% rate of polymorphism among eight tomato accessions. The most informative primers were B06 and H05 by revealing the maximum number of bands in the germplasm. The dendrogram for the morphological traits classified the germplasm into a single larger cluster except for genotype 17880. The dendrogram constructed for the molecular data clearly segregated the germplasm into two clusters. The accessions 17862 and 17870 were placed in the small cluster while the rest of the accessions were grouped together in the larger cluster. Genetic similarity ranged from 0.31 to 0.83 with an average of 0.68 for all evaluated accessions.
