The present study was conducted to evaluate the effects of chronic bisphenol A (BPA) exposure and the possible protective effects of polydatin (PD) on different organs, oxidant-antioxidant status and spermatological parameters in male Wistar rats. Male Wistar rats (2 to 3 months old) were randomly divided into 5 groups (n = 6). Group 1 was the control (daily 0.5ml distilled water). Groups 2, 3, 4 and 5 daily received 0.5 ml olive oil (OO), 25 mg/kg bisphenol A in 0.5 olive oil (BPA), 150 mg/kg polydatin (PD), and a mixture of 25 mg/kg bisphenol A in 0.5 olive oil + 150 mg/kg polydatin (BPA-PD), respectively, daily for 45 days. BPA had negative effects on percent abnormal sperm and Hypo-osmotic swelling-Eosin (HE) test (P<0.01). BPA caused negative histopathological alterations to the brain, heart, liver, kidney, and testis tissues. PD administration was able to ameliorate the negative effects of BPA. Moreover, PD had positive effects on percent motility (P<0.02), head defects of sperm (P<0.01), and HE test (P<0.01) when compared to the controls. The results indicate that PD decreases BPA-induced sperm defects and repaired tissue damage in the various organs in rats.
