Somporn Prasertsongskun and Naiyana Srichai

pp. 757 - 763

Abstract

The effects of different concentrations of growth regulators and activated charcoal on micropropagation were studied. Seedlings of orchid, Dendrobium friedericksianum Rchb.f, cultured on Vacin and Went
medium containing 1.0 mg/l α-naphthaleneacetic acid (NAA) and 1.0 mg/l kinetin (KN) significantly yielded
per cultured plant, the highest average number of leaves (4.40), roots (8.30), shoot length (1.67 centimeters)
and fresh weight (0.23 grams). Particularly when activated charcoal was added (0.3% w/v) the average
number of leaves, roots, shoot length and fresh weight per cultured plant increased. Percentage survival of
the plantlet after 21 days of transplantation was 85%.

Keywords

• Dendrobium friedericksianum Rchb.f
• growth regulator
• activated charcoal
• micropropagation