Pornthip Prommuang, Chongmas Antarasena and Naruepol Promkuntod

pp. 837 - 847

Abstract

The fluorescent conjugate for diagnosis of Newcastle disease was prepared in 6-week-old chickens by
immunization with Newcastle disease virus (NDV), strain F and local isolated strain NK1180/42. The antisera
with high neutralizing antibodies titers were collected and pooled.The immunoglobulin was fractionated and
conjugated with the fluorescein isothiocyanate (FITC). The conjugate was tested for the specificity in NDV
infected chorioallantoic membrane (CAM) and cryostat sections of tracheal epithelium, lung, heart, kidney,
spleen, liver, Bursa of Fabricius, brain and intestine. A distinctive fluorescence was seen in the cytoplasm of
infected cells of trachea, spleen, Bursa of Fabricius and intestine 48-72 hours after innoculation. The prepared conjugate was specific to NDV and diagnosis and result could be made in 1-2 hours.

Keywords

• Newcastle disease
• fluorescent conjugate
• chorioallantoic membrane
• experimental chickens