Sorawuth Bunthawin and Maneerat Boonlamp

pp. 393 - 416

Abstract

A spherical double shell model (SDM) for a single cell has been developed, using Laplace’s equation
in spherical coordinates and boundary conditions. Electric field intensities and dielectric constants of each
region inside and outside of the cell have been estimated. The dielectrophoretic spectrum of the real part of
a complex function (Re[f (ω)] were computed using Visual Foxpro Version 6, which gave calculated values
pertaining to electrical properties of the cell model as compared with experimental values. The process was
repeated until the error percentile was in an acceptable range. The calculated parameters were the dielectric
constants and the conductivities of the inner cytoplasm (ε_ic, σ_ic), the outer cytoplasm (ε_oc, σ_oc), the inner membrane (εim, σim), the outer membrane (ε_om, σ_om), the suspending solution(ε_s, σ_s) and the thickness of each
layer (d_om, d_oc, d_im), respectively. This computer program provides estimated values of cell electrical properties with high accuracy and required minimal computational time.

Keywords

• dielectric dispersion
• double-shell model
• dielectrophoresis
• computer program
• dielectric constant
• conductivity