Somchai Chuan-udom, Asalan Hilae and Luddawan Moosikapala

pp. 685 - 691

Abstract

Embryogenic callus of oil palm induced from young leaves of seedlings DxP was used as initial material
for protoplast isolation. Various combinations of cellulase Onozuka RS and macerozyme R-10 were tested.
Isolated protoplasts were cultured by various methods in MS medium supplemented with different phytohormones. The results revealed that 2% cellulase RS in combination with 2% macerozyme R-10 (adjusted
osmoticum to 0.4 M by manitol) yielded the highest number of viable protoplasts (1x10⁷
 per gram fresh
weight). Dicamba at concentration 2 mg/l with 1 mg/l 6-benzyladenin (BA) containing in phytagel semisolidified MS medium promoted the highest division of 2.3-4.0%. First division of the protoplasts was
observed at 4 days after culture. Microcolony formation (8-10 cells) was seen after three weeks of culture.
Unfortunately, neither callus formation nor plantlet regeneration were obtained.

Keywords

• oil palm
• embryogenic callus
• protoplast
• microcolony
• dicamba