Panote Thavarungkul and Kamnoon Kanchanapoom

pp. 283 - 291

Abstract

External currents of ± 2 µA were applied to the calluses of oil palm in three different culture medium
recipes containing different growth substances and the effects of the current were investigated. The three
media were medium for callus growth, embryogenetic medium, and organogenetic medium. The stimulation
of callus growth was found for both directions of current in one unit of experiments where the embryogenetic
culture medium contained NAA. In other cases the use of current seemed to have no significant stimulation
effect. The mechanism by which the current may cause the alignment of the cells to promote polar transport
of NAA which then increase growth and the relationship of these findings to earlier reports of the stimulation
of growth in tobacco callus cultures is discussed.

Keywords

• applied currents
• auxin
• oil palm
• polar transport
• tissue cultures