Lanchakorn Chan-udom, Sukanya Chanthachum and Aran H-Kittikul

pp. 817 - 824

Abstract

Coconut water and tuna condensate were used to produce bacteriocin from Lactobacillus casei ssp.
rhamnosus SN 11 by comparing with commercial medium, MRS. Bacteriocin activity was determined by
using Staphylococcus aureus as indicator. When L. casei ssp. rhamnosus SN 11 was grown in MRS medium
the highest bacteriocin was observed at 18-24 hour with inhibitory activity of 20 AU/ml. While growing in
modified media CW 1 (tuna condensate : coconut water = 1 : 1) the organism had inhibitory activity of
bacteriocin of 20 AU/ml at 20 hour. In modified medium CW 2 (1 : 2), CW 3 (1 : 3), and CW 4 (1 : 4) had
inhibitory activity of bacteriocin of 10 AU/ml at 16 hour. The bacteriocin activity was also found when L.
casei ssp. rhamnosus SN 11 grown in Tuna 2 (tuna condensate : coconut water = 2 : 1) and Tuna 3 (3 : 1) but
no activity was observed in Tuna 4 (4 : 1).

Keywords

• bacteriocin
• Lactobacillus casei ssp. rhamnosus SN 11