Alongkoad Tanomtong, Wiwat Kaensa and Roungvit Bunjongrat

pp. 753 - 764

Abstract

This is a karyotypic study of the masked palm civet, Paguma lavata (Carnivora, Viverridae). Blood samples were taken from one male and two females kept in Khao Khiew Open Zoo. After the standard whole blood lymphocyte culture at 37ºC for 72 h. in presence of colchicine, metaphase spreads were prepared on
microscopic slides and air-dried. Conventional staining and G-banding method were applied to stain the chromosome. The results showed that the number of diploid chromosomes of masked palm civet was 2n = 44, the fundamental number (NF) was 72 in the male and female. The type of autosomes were 2 large metacentric, 6 large submetacentric, 6 large acrocentric, 2 large telocentric, 2 medium metacentric, 2 medium submetacentric, 2 medium acrocentric, 8 medium telocentric, 2 small metacentric, 2 small submetacentric, 2 small acrocentric and 6 small telocentric chromosomes. In addition, there was a pair of chromosome 17 with clearly observable satellite chromosome. X-chromosome is a large submetacentric and Y chromosome is the smallest submetacentric chromosome. From the G-banding technique, the number of bands and
locations of G-band in masked palm civet was 141 and each chromosome pair could be clearly differentiated. The karyotype formula for the male and female masked palm civet is as follows: 2n (44) = L^m₂+L^sm₆+L^a₆+ L^t₂+M^m₂+M^sm₂+M^a₂+M^t₈+S^m₂+S^sm₂+S^a₂+S^t₆+ Sex-chromosome

Keywords

• karyotype
• masked palm civet (Paguma lavata)
• conventional staining
• G-banding