Alongkoad Tanomtong, Praween Supanuam and Sumpars Khunsook

pp. 421 - 426

Abstract

Cytogenetics of the pileated gibbon (Hylobates pileatus) at Nakhon Ratchasima Zoo, Thailand, was studied. Blood samples were taken from two female and two male gibbons. After lymphocyte culture, the mitotic chromosome preparation was done by hypotonic-fixation-air-drying method and conventional Giemsa’s staining. The results show that diploid chromosome number was 44 (2n=2x=44), and the fundamental number (NF) were 88 chromosomes in both female and male. The autosomes consist of 12 large metacentric, 6 medium metacentric, 2 medium submetacentric, 2 medium acrocentric, 12 small metacentric and 8 small submetacentric chromosomes. In addition, the chromosome 15 showed clearly observable
satellite chromosomes. The X chromosome was a medium submetacentric chromosome and the Y chromosome was a tiny acrocentric chromosome. The karyotype formula for the pileated gibbon is as follows:
2n (44) = L^m₁₂+M^m₆+M^sm₂+M^a₂+S^m₁₂+S^sm₈+sex-chromosomes

Keywords

• karyotype
• pileated gibbon (Hylobates pileatus)
• conventional staining