Ornubol Chomdej, Sukhuman Whankaew, Orawan Chatchawankanpanich, Wichai Kositratana and Julapark Chunwongse

pp. 441 - 446

Abstract

Resistance to tomato yellow leaf curl disease caused by Tomato yellow leaf curl Thailand virus (TYLCTHV-[2]) in wild tomato, Solanum habrochaites ‘L06112’ was investigated. The ‘L06112’ accession expressing the resistant phenotype was crossed to the TYLCV-susceptible female parent, Seedathip3, to produce F₁ hybrids. Parental polymorphism and hybrid identity were tested using 12 pairs of microsatellite markers for each chromosome. All markers were polymorphic between the parents, but only markers SSR46, SSR115, SSR117 and SSR128 gave results suitable to assess hybrid relationships. Polymorphic bands were sharp, concise and distinguishable between hybrids and selfed plants. The stem cuttings of donor and recurrent parents, their F₁and BC₁F₁ were inoculated with TYLCTHV-[2] using viruliferous whiteflies. Disease response of the plants was evaluated by Enzyme-Linked Immunosorbent Assay (ELISA) at 45 days post inoculation. The donor parental line showed complete resistance to TYLCTHV-[2] while the F₁ and BC₁F₁ expressed various ELISA readings for TYLCTHV-[2] concentration. BC₁F₁ ; 04T105-7, 04T105-1, 04T105-10, 04T109-4 and 04T104-1 developed from this study showed the high level of resistance to TYLCV, Thailand isolate.

Keywords

• Solanum habrochaites
• S. lycopersicum
• tomato yellow leaf curl disease
• TYLCTHV-[2]
• Breeding for disease resistance