Nithida Phonkot, Preeya Puangsomlee Wangsomnuk, and Chantana Aromdee

pp. 373 - 377

Abstract

A high-performance liquid chromatography method was developed to determine β-carotene content in lotus stamens.
The stamens of four varieties of Nelumbo nucifera (Gaertn.) and ten samples bought from traditional drug stores were determined. Validation of the method was carried out, the linearity of the β-carotene concentrations range from 6.50-58.50 μg.mL^-1
were 0.9997-0.9998. The repeatability and intermediate precision were 0.29 %CV (n=9) and 7.48 %CV (n=9), respectively; the
accuracy was 100.10 %w/w, the detection limit was 9.83 ng.mL^-1 and the quantitation limit was 29.80 ng.mL^-1. The β-carotene
of the four varieties were 465.77-1150.80 mg% (n=3), and of the store samples were 4.70-41.73 mg% (n=3). The results
demonstrated that the contents varied according to the variety. The contents in the samples obtained from traditional
drug stores were much lower that those dried in our laboratory, this might due to the source of the stamens, duration, and
conditions of storages.