Original Article |
2011, Vol.33, No.4, pp. 397-404
Effect of various pretreatment conditions on enzymatic saccharification
Muhammad Irfan, Muhammad Gulsher, Sajjad Abbas, Quratualain Syed, Muhammad Nadeem, and Shahjahan Baig
pp. 397 - 404
Abstract
The present work deals with the delignification of wheat straw and sugarcane bagasse with different pretreatment techniques followed by saccharification with commercial and indigenously produced cellulase enzymes. 100 g of sugar cane bagasse and whaet straw were treated with various concentrations of H2O2 (1-5% v/v) and its combination with 2% NaOH for one hour under steam at 30 psi. Sugarcane bagasse was 88% delignified with 5% H2O2 + 2% NaOH whereas wheat straw showed 51% delignification with 3% H2O2 + 2% NaOH. Moreover the saccharification with commercial cellulase enzyme was found to be 33.6% and 63.3% with pretreated wheat straw and sugarcane bagasse, respectively. However, very low levels of saccharification (6-14%) were found with indigenously produced cellulase enzyme.