Issoufou Amadou, Tidjani Amza, Yong-Hui Shi, and Guo-Wei Le

pp. 509 - 515

Abstract

The aim of this research was to extract phenolic compounds and assess the antioxidant potential of defatted foxtail
millet bran (DFMB) extracts. Distilled water, 30%, 50%, and 70% ethanol were used as solvent for extraction. The proximate
composition, fatty acid analysis, total phenolic content and extraction yield were carried out. The antioxidant efficacies of
DFMB extract were tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid
(ABTS), superoxide radical and reducing power. The highest inhibitory concentration (IC₅₀) values scavenging on DPPH,

ABTS and superoxide radicals were in the range of 0.131±0.002, 0.795±0.003, and 0.158±0.009 mg/ml, for distilled water, and 30
and 50% ethanol extracts respectively. The reducing power increased with increase of sample concentration. Total phenolic
content ranged from 21.49±3.26 to 29.39±1.36 gallic acid equivalent (GAE)/100 g extract. The phenolic content and antioxidant activity in DFMB extracts were significantly correlated (p<0.05). These results indicated that 50% ethanol extract from
DFMB appeared to be the most promoting phenolic compound with substantial antioxidant activity.