Optimization of process parameters for enhanced biodegradation of acid red 119 by Bacillus thuringiensis SRDD
Shailesh R. Dave and Riddhi H. Dave
pp. 23 - 30
Abstract
Developed Bacillus thuringiensis SRDD showed degradation of C.I. Acid red 119 and growth under the extreme condition of temperature 70°C, pH 3-8, heavy metals concentration of 0.8 mM, NaCl up to 900 mM and 1000 ppm dye. Cotton seed, caster cake and corn cake powders were found to be better and cheaper nutrient supplements for the Bacillus thuringiensis SRDD for biodegradation as compared to molasses. After development of the culture and the process, more than 99% degradation was achieved in less than 2 hrs of contact time even on 18th cycles of addition of 100 ppm AR-119 dye. The developed process showed AR-119 biodegradation rate as high as 220 mg L-1 h-1, which is found to be 130 times more as compared to the reported data. U.V., FTIR, TLC and HPLC analysis data confirmed biodegradation ability of the Bacillus thuringiensis for AR-119.