Kingkan Bunluepuech, Chatchai Wattanapiromsakul, and Supinya Tewtrakul

pp. 665 - 669

Abstract

The ethanol extract of heartwood from Cassia garrettiana had strong anti-HIV-1 integrase (IN) activity with an IC₅₀
value of 3.0 µg/mL. Therefore, its fractions and compounds were investigated for their anti-HIV-1 IN effect using the multiplate integration assay (MIA). From bioassay-guided isolation, the ethyl acetate fraction was then separated to give five
compounds which are chrysophanol (1), piceatannol (2), aloe-emodin (3), emodin (4) and cassigarol E (5). Of the tested
samples, piceatannol (2) showed the highest activity against HIV-1 IN with an IC₅₀ of 17.9 µM, followed by cassigarol E (5,
IC₅₀ = 72.9 µM), respectively. This is the first report on anti-HIV-1 IN activity of Cassia garrettiana.