Sukunlaya Senapong, Jindaporn Puripattanavong, and Rawee Teanpaisan

pp. 451 - 457

Abstract

This study aimed to investigate the anticandidal and antibiofilm activity of Artocarpus lakoocha extract against
various Candida. Anticandidal activity of A. lakoocha extract was determined using an agar well diffusion method. Minimum
inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were assessed using a method of the Clinical
and Laboratory Standards Institute. A time kill assay was also performed. Antibiofilm activity was investigated using a 3-[4, 5-
dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide (MTT) assay. The extract was found to be effective against all
tested Candida strains with MIC ranging from 0.05 to 3.12 mg/ml and MFC ranging from 0.10 to 25 mg/ml. The killing activity
depended on the time and concentrations of the extract. A. lakoocha extract acts as a potent antibiofilm agent with dual
actions, preventing and eradicating the biofilm. Results suggest that A. lakoocha extract is a potential source of natural
anticandidal agent, which may be useful for prevention or treatment of candidiasis.