Malinee Wongnawa, Ruhainee Tohkayomatee, Nisita Bumrungwong, and Sumpun Wongnawa

pp. 541 - 546

Abstract

This study investigated the α-glucosidase inhibitory effect and determined the concentration of some inorganic
constituents in P. amarus ash. Oral glucose and sucrose tolerance test were performed on normal mice. In vitro α-glucosidase
inhibitory activity was evaluated by using yeast a-glucosidase. The element concentrations were measured by inductively
coupled plasma (ICP) spectroscopy. Single oral administration of P. amarus ash did not show antihyperglycemic effect after
glucose administration, but decreased blood glucose level after sucrose administration. The ash showed α-glucosidase
inhibitory activity in vitro with IC₅₀ of 982 mg/mL. The concentrations of K, Ca, Mg, Mn, Fe, Zn, Cu, Pb, Cr, Ni and Co in
P. amarus ash were 35049.80±340.64, 3337.24±52.10, 1368.52±13.29, 90.81±1.34, 87.68±1.15, 18.28±0.22, 4.69±0.07, 1.07±0.15,
0.29±0.03, 0.20±0.04 and 0.10±0.02 mg/g, respectively. These results indicate that the antihyperglycemic effect of P. amarus
ash might be partly due to the α-glucosidase inhibitory activity of the inorganic constituents.