Nisaudah Radenahmad, Kitja Sawangjaroen, Winyou Mitranun, and Ibrahim Sayoh

DOI: 10.14456/sjst-psu.2016.8

pp. 57 - 65

Abstract

The purpose of this study was to determine the effects of feeding young coconut juice (YCJ), known to contain ß-
sitosterol, to ovariectomized rats, a model for postmenopausal women, on the lipid, renal and liver metabolism profiles. Four
groups of female rats (6 in each group) were included in this study. These included sham-operated, ovariectomized (ovx),
ovx receiving estradiol benzoate (EB) injections intraperitoneally, and ovx receiving YCJ orally. At the end of the third and
the fourth week of study, the rats were sacrificed and their serum estradiol (E2) was analyzed. The uterus was removed along
with the kidney and liver. The latter was paraffin processed for histopathological assessment. In contast with the 7 days
treatments, all parameters of the 14 days treatment had improved. After 14 days of treatment, the circulating levels of BUN,
creatinine, cholesterol, triglyceride, LDL, AST, ALT, ALP, total protein and albumin of ovx+YCJ group were not significantly
different from the sham and ovx groups. Only the serum HDL level of the ovx+YCJ group was significantly higher than that
of the sham group. The histopathological assessment of the liver and kidney showed no significant changes when compared
with the control groups. Glycogen accumulation appeared in the cytoplasm of the hepatocytes particularly in the ovx+YCJ
group but significant changes of the hepatocytes containing glycogen were not detected. No other abnormal features were
seen in any of the four groups. The %age uterine/body weights indicated that YCJ feeding in ovx rats at 100 mL/kgBW for
up to two weeks did not cause increased uterine weight. In summary, this study confirmed that feeding YCJ had beneficial
effects on the serum lipid profile, and maintained liver and renal functions for up to 2 weeks after administration.