Histological organization of the female queen Devario regina (Fowler, 1934) during its juvenile stage
Piyakorn Boonyoung, Sinlapachai Senarat, Jes Kettratad, Pisit Poolprasert, Watiporn Yenchum, and Waristha Angsirijinda
pp. 67 - 72
Abstract
Limited research has been reported in the basic information about the structural organizations of fish organs in their
juvenile state that could be used as histopathological biomarkers. Thus, the histological structures of important organs of
the female fish Devario regina (Fowler, 1934) during its juvenile stage were exclusively examined using histological and
histochemical approaches. Specimens were collected during the fishing season (July and October 2010) from the Tapee river,
Thailand. Using histological analysis, the digestive system was distinctly composed of two parts; the digestive tract and
accessory organs (liver and pancreas). Based on their histological structure, the epithelial organization of the oral cavity and
pharynx was lined by stratified epithelium whereas the intestine was covered by a simple columnar epithelium and contained
several goblet cells. The goblet cells were negatively stained with hematoxylin and eosin (H&E) and Masson’s Trichrome
(MT). In contrast, they were positively stained with Periodic Acid Schiff reaction (PAS) and aniline blue (AB). The liver tissue
in this fish was composed of polyhedral hepatocytes, with their sinusoids being distinctly located between the hepatocytes.
The sinusoids were lined by a simple squamous epithelium. The pancreatic parenchyma mainly consisted of pyramidal cells
that rested on a basal lamina in the acinar. Moreover, the pancreatic cells had a basophilic cytoplasm, a distinct basal nucleus
and contained large eosinophilic zymogen granules. The excretory system especially referred to the kidney, and was composed
of renal tubules and hematopoietic tissue. The female reproductive system was the ovary that was surrounded by a tunica
albuginea. The ovary contained oocytes at differential stages of development including oogonia and the previtellogenic stage.
Finally, the integument of this species consisted mainly of three layers of epidermis, dermis and hypodermis, respectively
