R. Susanti

DOI: 10.14456/sjst-psu.2016.18

pp. 137 - 142

Abstract

In Indonesia, the outbreaks of highly pathogenic avian influenza (HPAI) have been including in domestic poultry
since 2006. In this study, the pathogenicity of two isolates of H5N1 (A/goose/Bojonggenteng/IPB2-RS and A/duck/Parung/
IPB8-RS), which had been isolated from waterfowls, was assessed in chickens and Balb-c mice. The egg infectious dose EID₅₀
results were high in chickens. The virus killed the chickens within 24 hours after post infection and it showed maximum IVPI
value. The virus antigens were detected in chicken internal organs. Further assessment using a biological test in Balb-c mice
was performed to determine the infection effectiveness and pathogenicity phenotype. Two virus isolates were highly pathogenic and the viruses were detected in the mice core organs. This result indicated that the virus was able to replicate in
mammals. Moreover, the avian influenza viruses were found genetically and biologically pathogenic.