Nararatn Wattanapan, Charassri Nualsri, and Upatham Meesawat

DOI: 10.14456/sjst-psu.2018.48

pp. 306 - 313

Abstract

Paphiopedilum callosum var. sublaeve, a lady’s slipper orchid native to southern Thailand, is protected under CITES
(appendix I) due to habitat destruction and over-collection. The novel micropropagation technique for this endemic species was
investigated. A 2-week preculture of seeds in distilled water before transfer to 1/2MS solid medium was the optimal conditions to
promote seed germination in vitro. Shoot tip-derived transverse thin cell layer (tTCL) explants cultured on MVW solid medium
containing 1.0 mg/L TDZ for 8 weeks provided the highest percentage of regenerated protocorm-like bodies (46.67±6.67), shoot
formation (40.00±5.16), root formation (30.00±12.38) and survival rate (70.00±4.47). The acclimatized plantlets potted in
sphagnum moss in the greenhouse grew well at 80% survival rate. No genetic variation was detected between the regenerated
plantlets and their own mother plants based on RAPD marker