Pilaiwan Siripurkpong and Mudtika Fungkrajai

DOI: 10.14456/sjst-psu.2020.126

pp. 975 - 983

Abstract

The present study was aimed to determine the effects of Torvoside A from methanolic extract of Solanum torvum on
adipogenesis, fat accumulation, and adiponectin synthesis as well as glucose uptake activities. For adipogenesis assay, 3T3-L1-
preadipocytes were treated with Torvoside A (0, 5, 25, 50 µM) during 7 days of adipocyte differentiation, while 3T3-L1 mature
adipocytes were treated with Torvoside A for 2 days in the fat accumulation study. There were no significant differences in the
intracellular triglyceride among Torvoside A treated cells and the untreated control (p>0.05) in both assays. Torvoside A did not
significantly change adiponectin synthesis in both transcriptional and translational levels (p>0.05), measured by real-time RTPCR and western blot, respectively. Finally, Torvoside A did not affect glucose uptake activity and GLUT4 transcript as well.
These results can be concluded that Torvoside A could not directly promote adipocyte hyperplasia and hypertrophy as well as the
anti-diabetic activities.