Udomlak Sukatta, Prapassorn Rugthaworn, Nattaporn Khanoonkon, Pariya Anongjanya, Kunat Kongsin, Prakit Sukyai, Nathdanai Harnkarnsujarit, Rungsinee Sothornvit, and Rungsima Chollakup

DOI: 10.14456/sjst-psu.2021.6

pp. 37 - 44

Abstract

This study focused on assessment of the bioactivities and characterization of rambutan peel extract (RPE) and the
feasibility of RPE as a bioactive compound for antimicrobial and antioxidant applications in whey protein isolate (WPI)/cellulose
nanocrystal film. The RPE inhibited against nine strains of pathogenic and food spoilage bacteria (Micrococcus luteus, Bacillus
sp., Escherichia coli, Pseudomonas fluorescens, Salmonella typhimurium, Staphylococcus aureus, Enterococcus faecalis,
Streptococcus sp. and Staphylococcus aureus). The extract also exhibited higher antioxidant activities than those of butylated
hydroxytoluene (BHT) (via DPPH, ABTS and FRAP assays), α-tocopherol (via DPPH and ABTS assays) and butylated
hydroxyanisole (BHA) (via hydroxyl radical and superoxide anion radical scavenging assays). The WPI/cellulose nanocrystal
film containing 3% RPE exhibited moderate antimicrobial activity and strong antioxidant activity, which can be applied as a
bioactive film for food packaging application.