Abdoreza Aghajani, Seyed Ali Mortazavi, Farideh Tabatabaei Yazdi, Masoud Shafafi Zenozian, and Mohammad Reza Saeidi Asl

DOI: 10.14456/sjst-psu.2020.2

pp. 8 - 14

Abstract

In this study, response surface methodology (RSM) was used to optimize the conditions for the extraction of total
flavonoid content (TFC) and determine the free radical scavenging activity (IC₅₀ value) from Ferulago angulata extract by
ultrasound bath at constant temperature. The investigated factors in this method were the solvent ratio (ethanol/water at 0 to
100%) and extraction time (4–8 min). Experiments were carried out based on a central composite design. The results showed that
the extraction conditions had a significant effect on the IC₅₀ value and TFC extraction. The optimal conditions for TFC (0.56
mgRE/g) included ethanol content and extraction time and were determined to be 49.49% (v/v) and 7 min and 39 sec,
respectively. Furthermore, the IC₅₀ value (1.13 mg/g) was 98.27% (v/v) and 8 min. It was also found that RSM in optimizing
extraction conditions, especially in predicting TFC and IC₅₀ values, is an efficient method. These optimized conditions allow the
rapid and maximum extraction of bioactive compounds from F. angulata.