Witsarut Kraithong, Wipawee Juengsanguanpornsuk, Supaluk Krittanai, Gorawit Yusakul, and Waraporn Putalun

DOI: 10.14456/sjst-psu.2021.93

pp. 703 - 710

Abstract

In vitro cultures of Pueraria candollei var. mirifica (PC) are a promising source of phytoestrogens, however, the
estrogenic activity of PC callus has not been evaluated. In this study, the phytochemicals of PC callus were analyzed and
compared with the estrogenic activities on MCF-7 cell proliferation. The ethanolic extract of PC callus contained isoflavonoids
and deoxymiroestrol. The estrogenic activity of the extract (10 µg/mL) was equivalent to 1×10^-10 M estradiol. When the crude
extract was reconstituted with 10% (v/v) ethanol in water, most of the isoflavonoid and deoxymiroestrol contents were
solubilized. The soluble fraction exhibited the estrogenic effect in a concentration-dependent manner, but treatment with the
insoluble fraction at a high concentration (100 µg/mL) resulted in cell growth suppression. Overall, the crude ethanolic extract
and 10 %(v/v) ethanol-soluble crude extract of PC callus were good sources of phytoestrogens. The reconstitution could remove
toxic constituents, but the estrogenic activity was retained.