Agung Irawan, Cuk Tri Noviandi, Kustantinah, Budi Prasetyo Widyobroto, and Andriyani Astuti

DOI: 10.14456/sjst-psu.2021.156

pp. 1197 - 1203

Abstract

This study evaluated the effects of Leucaena leucocephala and corn oil supplemented to diet on in vitro ruminal and
post-ruminal nutrient degradation using a two-stage in vitro technique. The four dietary treatments consisted of Pennisetum
purpureum and concentrate in a 75:25 ratio with increasing levels of L. leucocephala (L0= 0%, L25= 25%, L50= 50%, and L75=
75%, DM basis) with three levels of corn oil (CO) (CO1= 0%, CO2= 1%, and CO3= 2% of the substrate) resulting in 12
treatment combinations that were randomly assigned to a 4×3 factorial design. The L75 and CO3 treatment decreased ruminal
and total dry matter and organic matter degradation (p<0.001). Leucaena supplementation also increased ruminal and post
ruminal protein degradation (rdN and pdN) while CO decreased the rdN and the pdN. To conclude, inclusion of Leucaena to the
diet by 50% improved nutrients degradation, particularly ruminal and post-ruminal N degradation, while CO at 1% level
negatively affected nutrient degradation in the rumen.