The study aimed to preliminarily explore the relationship of the rubber tree’s physiological status and technological properties with response to different agroecosystem components, especially leaf area index (LAI) and soil moisture contents (SMC) under three types of rubber-based intercropping, notably rubber-bamboo (RB), rubber-coffee (RC) and rubber-melinjo (RM) compared to a rubber monocrop (R). RB and RM significantly showed the highest LAI values of around 1.4, while that of R had the smallest value (about 30% lesser than the highest values). RM, RB dramatically increased the SMC with the soil depths. The rubber-based intercropping farms indicated better biochemical composition in the latex, showing efficient metabolism of the latex biosynthesis. Technological properties of the raw rubber from the rubber-based intercropping farms expressed the premium results in both non-rubber components and rheological properties with higher molecular weights. The correlation analysis revealed some significant relations among the LAI, SMC, biochemical composition and technological properties.